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 Add this item to the list   Phytophthora alni subsp. alni
    

General information

 Organism group:Phytophthora 
 Taxonomic info on species (MycoBank):
 
 Representative strain(s):PD 20019550 = P772 = P16203 
 Associated strain(s) in Q-bank:
 

Substrate and country information

 Substrates of included strains:Alnus glutinosa (Betulaceae); Alnus sp. (Betulaceae) bark 
 Countries of included strains:Hungary; United Kingdom 
 
Other reported substrates and countries
 
Kingdom Planta
 Plant host genus:
Alnus
 
 
Symptoms on host plant:
antracnose no
canker no
dieback no
wilting no
 
leaf spot no
root rot yes
stem base rot no
   
 
 
Type of infection on plant:
Pathogenic yes
Opportunistic no
Saprophytic no
 
 

Quarantine information

 Has quarantine status?:No 
 EU status:
 
 EPPO status:
 

Morphological data

 
Pictures
 Culture CMA after 7 days at 24 °C:
  • Phytophthora alni subsp. alni CBS 117375 CMA after 7 days at 24 degrees
 
 Culture PDA after 7 days at 24 °C:
  • Phytophthora alni subsp. alni CBS 117375 PDA after 7 days at 24 degrees
 
 Culture V8 after 7 days at 24 °C:
  • Phytophthora alni subsp. alni CBS 117375 V8 after 7 days at 24 degrees
 
 Sporangia:
  • Phytophthora alni subsp. alni P16203 obpyriform sporangium 100x
  • Phytophthora alni subsp. alni P16203 sporangium 100x
  • Phytophthora alni subsp. alni P16203 sporangium 100x
  • Phytophthora alni subsp. alni P16203 sporangium 100x
 
 Sporangiophores:
  • Phytophthora alni subsp. alni P772 internal nested proliferation 2 100x
  • Phytophthora alni subsp. alni P772 internal proliferation 3 100x
  • Phytophthora alni subsp. alni P772 sporangia internal proliferation 100x
  • Phytophthora alni subsp. alni P772 sympodial branching 100x
 
 Oogonia & Antheridia:
  • Phytophthora alni subsp. alni CBS 117375 oogonia antheridia 2 100x
  • Phytophthora alni subsp. alni CBS 117375 oogonium antheridium 1 100x
  • Phytophthora alni subsp. alni CBS 117375 oogonium ornamented 1 100x
  • Phytophthora alni subsp. alni CBS 117375 oogonium ornamented 2 100x
  • Phytophthora alni subsp. alni P16203 oogonium ornamented 1 100x
  • Phytophthora alni subsp. alni P16203 oogonium ornamented 2 100x
  • Phytophthora alni subsp. alni P16203 oogonium ornamented 3 100x
  • Phytophthora alni subsp. alni P16203 ornamented oogonium 100x
  • Phytophthora alni subsp. alni P16203 ornamented oogonium two celled antheridium 100x
  • Phytophthora alni subsp. alni P16203 overview of typical oogonia 100x
 
 Culture CMA after 7 days at 18 °C:
  • Phytophthora alni subsp. alni P772 CMA after 7 days at 18 degrees
 
 Culture PDA after 7 days at 18 °C:
  • Phytophthora alni subsp. alni CBS 117375 PDA after 7 days at 18 degrees
 
 Culture V8 after 7 days at 18 °C:
  • Phytophthora alni subsp. alni CBS 117375 V8 after 7 days at 18 degrees
 
 
Colony morphology
 
CMA, colony pattern description:
no special pattern yes
rozette no
 
chrysanthenum no
coarsly radiate no
 
 
PDA: colony pattern description:
no special pattern yes
rozette no
 
chrysanthenum no
coarsly radiate no
 
 
CMA colony edge description:
sharp no
vague, gradual yes
irregular, (rafelig) no
 
 
 
CMA: aerial mycelium description:
only submerged mycelium no
sparse aerial mycelium no
dense aerial mycelium yes
 
fluffy yes
velvety no
cottony no
 
 
PDA: aerial mycelium description:
only submerged mycelium no
sparse aerial mycelium no
dense aerial mycelium yes
 
fluffy yes
velvety no
cottony no
 
 
PDA: colony edge description:
sharp no
vague (gradual) yes
irregular, (rafelig) no
 
 
 
V8: colony pattern description:
no special pattern yes
rozette no
 
chrysanthemum no
coarsely radiate no
 
 
V8: colony edge description:
sharp no
vague, gradual yes
irregular (rafelig) no
 
 
 
V8: aerial mycelium description:
only submerged mycelium no
sparse aerial mycelium no
dense aerial mycelium yes
 
fluffy yes
velvety no
cottony no
 
 
Characters in vitro
 
Sporangia in water culture
 
Shape of sporangia:
spherical/globose yes
ellipsoid no
ovoid yes
obpyriform yes
obturbinate no
 
obovoid no
elongated neck/ beaked no
tapered base no
distorted shapes no
constricted ?
 
 Width of sporangia (µm):(19.4) 30.59 - 38.85 (45.59) 
 Average length/width ratio:(1.1) 1.2 - 1.48 (1.51) 
 Length of sporangia (µm):(22.31) 38.59 - 53.63 (64.51) 
 
Pore type of sporangia:
non-papillate yes
semi-papillate no
papillate no
 
 
 Discharge pore width:(9.51) 9.87 - 17.12 (19.4) 
 
Sporangial characteristics:
Are the sporangia caduceous? no
>1 apex/papilla no
Presence of hyphal projections (Oc) no
Collapse after dehiscence ?
 
Laterally displaced attachment of sporangiophore (Oc) no
Occurence of intercallary sporangia no
Basal plug conspicious yes
   
 
 
Sporangiophores in water culture
 
Sporangiophores on agar:
compound sympodial sporangiophores no
sympodial branching on agar no
are sporangia present on agar?: no
 
 
 
Sporangiophores in water:
long, irregularly branched yes
umbellate no
internal proliferation yes
Sympodia yes
 
basal swellings no
intercalary swellings yes
clumps no
   
 
 
Antheridia on CMA medium
 Length of antheridia (µm):(12.61) 13.58 - 22.5 (24.25) 
 Remarks on antheridia:
Two antheridia per oogonium do occur, The first oogonium always amphyginous, the second antheridium is paragynous and can be either diclinous or monoclinous.
2-celled antheridia are very common.
 
 
Shape of antheridia:
short cylindrical variable
elongated cylindrical variable
spherical yes
irregular no
 
rectangular no
ellipsoid or ovoid yes
club shaped no
   
 
 Width of antheridia (µm):(12.32) 13.58 - 20.7 (20.8) 
 
Type of antheridia:
amphigynous yes
paragynous variable
 
 
 
Antheridial characteristics:
Can antheridia be diclinous? variable
Do 2 celled antheridia occur? yes
Tangled or knotted hyphae around the antheridium no
 
Spine or digitate projections on antheridium variable
Can oogonia have 2 or more antheridia variable
Intercalary or subterminal antheridia? yes
 
 
Oogonia on CMA medium
 Length of oogonia (µm):(23.6) 37.73 - 55.33 (62.08) 
 Width of oogonia (µm):(23.6) 33.88 - 53.92 (58.2) 
 Remarks on oogonia:
P772: Ornamentation is present on all oogonia. excentric oogonial stalks within the antheridium are rare.
 
 Oogonia wall thickness (µm):(1) 1.18 - 2.23 (2.6) 
 
Oogonial characteristics:
Ornamentation yes
Tapered base yes
Wall rough with age no
 
Oogonial stalk often excentric in antheridium no
Clustered typically no
   
 
 
Oospores on CMA medium
 Width of oospores (µm):(18.5) 29.26 - 48.1 (51.7) 
 Wall thickness of oospores (µm):(0.39) 0.49 - 2.84 (3.7) 
 Length of oospores (µm):(18.5) 28.83 - 48.1 (51.7) 
 
Oospores plerotic or aplerotic?:
Plerotic yes
Aplerotic variable
Slightly aplerotic yes
 
 
 
Chlamydospores in water culture or on CMA medium
 Chlamydospores presence:absent 
 
Hyphal swellings in water culture
 
Presence of hyphal swellings:
In agar no
On agar no
 
In water no
Absent yes
 
 
Sexual state:
homothallic yes
heterothallic mating type A1 no
 
heterothallic mating type A2 no
Sterile no
 
 
Hyphae on CMA medium
 Maximum hyphal diameter:8 
 
Daily growth
 Daily growth at 24 °C on CMA agar in mm:3.8 
 
Cardinal temperatures
 Lowest temperature at which sustained growth was observed:3 
 Highest temperature at which sustained growth was observed:30 
 Optimum growth temperature interval starts at ... °C:18 
 Optimum growth temperature interval ends at ... °C:24 

DNA sequence data

 Sequences in Q-bank:
 

Literature

 Original publication:
 
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