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 Add this item to the list   Phytophthora kernoviae
    

General information

 Organism group:Phytophthora 
 Taxonomic info on species (MycoBank):
 
 Representative strain(s):CBS 122208 = CMW 31065 = PD 05 02010595 
 Associated strain(s) in Q-bank:
 

Substrate and country information

 Substrates of included strains:Magnolia sp. (Magnoliaceae); Rhododendron ponticum (Ericaceae); Rhododendron ponticum (Ericaceae) leaf 
 Countries of included strains:United Kingdom 
 
Other reported substrates and countries
 
Kingdom Planta
 Plant host family:Rhododendron 
 Plant host genus:ponticum 
 
Symptoms on host plant:
antracnose ?
canker yes
dieback yes
wilting ?
 
leaf spot yes
root rot no
stem base rot yes
   
 
 
Type of infection on plant:
Pathogenic yes
Opportunistic no
Saprophytic no
 
 
 Host symptoms:
  • Phytophthora kernoviae bleeding canker on Fagus sylvatica
  • Phytophthora kernoviae leaf infection on Rhododendron
  • Phytophthora kernoviae on Rhododendron
  • Phytophthora kernoviae on Rhododendron and Fagus sylvatica
  • Phytophthora kernoviae on Vaccinium 1
  • Phytophthora kernoviae on Vaccinium 2
  • Phytophthora kernoviae on severely infected Rhododendron
  • Phytophthora kernoviae severe infection on Rhododendron
  • Phytophthora kernoviae stem lesions on Vaccinium
 
 
Other substrata
 
Geographic origin
 Latitude, longitude of isolation:
(?°; ?°; ?) ± ? km (Hide map)
 

Quarantine information

 Has quarantine status?:Yes 
 EU status:
 
 EPPO status:
 
 EPPO (Bayer) code:PHYTKE 
 Q-species description sheet:
 
 Q-species distribution map:
 
 Q-species diagnostic protocol:
 
 Diagnostic locus for identification in Q-bank:ITS nrDNA sufficient; see Molecular Decision Scheme. 

Morphological data

 
Pictures
 Culture CMA after 7 days at 24 °C:
  • Phytophthora kernoviae CBS 122208 CMA after 7 days at 24 degrees
 
 Culture PDA after 7 days at 24 °C:
  • Phytophthora kernoviae CBS 122208 PDA after 7 days at 24 degrees
 
 Culture V8 after 7 days at 24 °C:
  • Phytophthora kernoviae CBS 122208 V8 after 7 days at 24 degrees
 
 Sporangia:
  • Phytophthora kernoviae CBS 122208 asymmetrical sporangium 100x
  • Phytophthora kernoviae CBS 122208 extremely papillate caducuous aborted sporangia 100x
  • Phytophthora kernoviae CBS 122208 sporangium 1 100x
  • Phytophthora kernoviae CBS 122208 sporangium 2 100x
  • Phytophthora kernoviae CBS 122208 sporangium 3 100x
  • Phytophthora kernoviae CBS 122208 sporangium on sporangiophore 100x
 
 Oogonia & Antheridia:
  • Phytophthora kernoviae CBS 122208 not readily formed oogonia 1 celled antheridia 2 100x
  • Phytophthora kernoviae CBS 122208 not readily formed oogonia 1celled antheridia 1 100x
  • Phytophthora kernoviae CBS 122208 oogonia antheridia 1 100x
  • Phytophthora kernoviae CBS 122208 oogonia antheridia 2 100x
  • Phytophthora kernoviae CBS 122208 oogonia antheridia 3 100x
  • Phytophthora kernoviae CBS 122208 oogonium 1celled antheridia 2nd hyphae 100x
  • Phytophthora kernoviae CBS 122208 sexual structure amphigynous 1 100x
  • Phytophthora kernoviae CBS 122208 sexual structure amphigynous 2 100x
  • Phytophthora kernoviae CBS 122208 sexual structure amphigynous 5 100x
  • Phytophthora kernoviae CBS 122208 sexual structure paragynous 1 100x
  • Phytophthora kernoviae CBS 122208 sexual structure paragynous 2 100x
 
 Culture CMA after 7 days at 18 °C:
  • Phytophthora kernoviae CBS 122208 CMA after 7 days at 18 degrees
  • Phytophthora kernoviae CBS 122208 CMA after 7 days at 18 degrees
 
 Culture PDA after 7 days at 18 °C:
  • Phytophthora kernoviae CBS 122208 PDA after 7 days at 18 degrees
  • Phytophthora kernoviae CBS 122208 PDA after 7 days at 18 degrees
 
 Culture V8 after 7 days at 18 °C:
  • Phytophthora kernoviae CBS 122208 V8 after 7 days at 18 degrees
  • Phytophthora kernoviae CBS 122208 V8 after 7 days at 18 degrees
 
 
Colony morphology
 
CMA, colony pattern description:
no special pattern no
rozette no
 
chrysanthenum no
coarsly radiate no
 
 
PDA: colony pattern description:
no special pattern no
rozette yes
 
chrysanthenum no
coarsly radiate no
 
 
CMA colony edge description:
sharp no
vague, gradual no
irregular, (rafelig) yes
 
 
 
CMA: aerial mycelium description:
only submerged mycelium no
sparse aerial mycelium yes
dense aerial mycelium no
 
fluffy yes
velvety no
cottony no
 
 
PDA: aerial mycelium description:
only submerged mycelium no
sparse aerial mycelium variable
dense aerial mycelium yes
 
fluffy no
velvety yes
cottony no
 
 
PDA: colony edge description:
sharp no
vague (gradual) no
irregular, (rafelig) yes
 
 
 
V8: colony pattern description:
no special pattern no
rozette yes
 
chrysanthemum yes
coarsely radiate no
 
 
V8: colony edge description:
sharp yes
vague, gradual no
irregular (rafelig) no
 
 
 
V8: aerial mycelium description:
only submerged mycelium no
sparse aerial mycelium yes
dense aerial mycelium variable
 
fluffy no
velvety yes
cottony no
 
 
Characters in vitro
 
Sporangia in water culture
 
Shape of sporangia:
spherical/globose no
ellipsoid yes
ovoid yes
obpyriform yes
obturbinate no
 
obovoid no
elongated neck/ beaked no
tapered base variable
distorted shapes variable
constricted no
 
 Width of sporangia (µm):(12.3) 18.7 - 28.7 (31) 
 Average length/width ratio:(1.2) 1.4 - 2.1 (2.3) 
 Caducous pedicels length (µm):(3.1) 4.4 - 16.8 (27.5) 
 Length of sporangia (µm):(19.2) 28.1 - 55.2 (62.3) 
 
Pore type of sporangia:
non-papillate no
semi-papillate no
papillate yes
 
 
 Discharge pore width:(3.1) 3.98 - 5.34 (6.6) 
 
Sporangial characteristics:
Are the sporangia caduceous? yes
>1 apex/papilla no
Presence of hyphal projections (Oc) no
Collapse after dehiscence no
 
Laterally displaced attachment of sporangiophore (Oc) no
Occurence of intercallary sporangia no
Basal plug conspicious variable
   
 
 
Sporangiophores in water culture
 
Sporangiophores on agar:
compound sympodial sporangiophores no
sympodial branching on agar yes
are sporangia present on agar?: yes
 
 
 
Sporangiophores in water:
long, irregularly branched no
umbellate no
internal proliferation no
Sympodia yes
 
basal swellings yes
intercalary swellings yes
clumps no
   
 
 
Antheridia on CMA medium
 Length of antheridia (µm):(5.82) 8.98 - 12.97 (15.04) 
 
Shape of antheridia:
short cylindrical yes
elongated cylindrical yes
spherical yes
irregular no
 
rectangular no
ellipsoid or ovoid yes
club shaped no
   
 
 Width of antheridia (µm):(7.08) 8.71 - 10.67 (11.06) 
 
Type of antheridia:
amphigynous yes
paragynous variable
 
 
 
Antheridial characteristics:
Can antheridia be diclinous? yes
Do 2 celled antheridia occur? yes
Tangled or knotted hyphae around the antheridium no
 
Spine or digitate projections on antheridium no
Can oogonia have 2 or more antheridia yes
Intercalary or subterminal antheridia? yes
 
 
Oogonia on CMA medium
 Length of oogonia (µm):(21.63) 24.25 - 28.84 (29.78) 
 Width of oogonia (µm):(19.59) 24.21 - 30.49 (31.04) 
 Oogonia wall thickness (µm):(0.5) 0.6 - 1.2 (1.5) 
 
Oogonial characteristics:
Ornamentation no
Tapered base yes
Wall rough with age no
 
Oogonial stalk often excentric in antheridium variable
Clustered typically no
   
 
 
Oospores on CMA medium
 Width of oospores (µm):(18.53) 22.23 - 28.14 (28.81) 
 Wall thickness of oospores (µm):(1.07) 1.16 - 2.74 (3.01) 
 Length of oospores (µm):(18.04) 21.53 - 27.08 (28.23) 
 
Oospores plerotic or aplerotic?:
Plerotic yes
Aplerotic no
Slightly aplerotic yes
 
 
 
Chlamydospores in water culture or on CMA medium
 Chlamydospores presence:absent 
 Length of chlamydospores (µm):? 
 Width of chlamydospores (µm):? 
 
Shape of chlamydospores:
globose ?
subglobose ?
Botryose ?
 
 
 Location of chlamydospores on plate:? 
 
Chlamydospore position:
Lateral ?
Terminal ?
Clustered ?
 
Intercallary ?
Catenulate ?
   
 
 Chlamydospore wall thickness:? 
 
Hyphal swellings in water culture
 
Shape of hyphal swellings:
Globose ?
radiating hyphae ?
Subglobose ?
 
Flask- or Bottleshaped / (Ob)-pyriform ?
Elongate ?
Irregular/coralloid ?
 
 Length of hyphal swellings (µm):? 
 Width of hyphal swellings (µm):? 
 
Clustering of hyphal swellings:
Solitary ?
Catenulate, in chains ?
Clustered / Aggregated ?
 
 
 
Presence of hyphal swellings:
In agar no
On agar no
 
In water no
Absent yes
 
 
Sexual state:
homothallic yes
heterothallic mating type A1 no
 
heterothallic mating type A2 no
Sterile no
 
 
Hyphae on CMA medium
 Maximum hyphal diameter:? 
 
Daily growth
 Daily growth at 24 °C on CMA agar in mm:0.87 
 
Cardinal temperatures
 Lowest temperature at which sustained growth was observed:3 
 Highest temperature at which sustained growth was observed:24 
 Optimum growth temperature interval starts at ... °C:21 
 Optimum growth temperature interval ends at ... °C:21 

DNA sequence data

 Sequences in Q-bank:
 

Literature

 Original publication:
 
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